Helicos® Genetic Analysis System Performance Specifications SEQUENCING PERFORMANCE AS OF MARCH 1, 2010   Routine Usage Specifications1   Strand Output 600M to 1B usable strands per run 12 to 20M usable strands per channel Sample Throughput Multiplexing capabilities up to 96 samples per channel with molecular barcoding.  Up to 4,800 samples per run. Total Output 21 to 35 Gigabases per run 420 to 700 Megabases per channel Read Length 25 to 55 bases in length 35 average length Accuracy >99.995% consensus accuracy at >20X coverage Raw Error Rate Substitution 0.2% Insertion 1.5% Deletion 3.0% Template Size 20 to 8,000 bases % GC Coverage <20% coverage variation for 20 - 80% GC (E. coli)   Instrument Performance / Configuration Flow Cells 1 flow cell or 2 flow cells processed simultaneously 25 independent channels per flow cell Sequencer Throughput > 1 Gigabases per hour2 1 Routine usage specifications based on genomic DNA samples loaded at recommended concentrations and 30 Quad run (8 days) 2 The imaging system is designed for 1 Gigabase/hour throughput performance. Actual sequencing performance is determined by chemistry efficiency and loading density