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tSMS™ Performance

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Helicos® Genetic Analysis System Performance Specifications

Instrument Performance / Configuration
Flow Cells 2 flow cells processed simultaneously
50 independent channels are available in 2 flow cells
Sample Throughput 1 – 100,000+ samples per run1
Sequencer Throughput > 1 Gigabases per hour2
SEQUENCING PERFORMANCE AS OF December 1, 2008
  Routine Usage Specifications3
Strand Output

12 to 16M usable strands per channel4

600 to 800M usable strands per run

Total Output

420 to 560 Megabases per channel

21 to 28 Gigabases per run

Throughput 105 to 140 Megabases per hour
Read Length

25 to 55 bases in length

30 to 35 average length

Accuracy >99.995% consensus accuracy at >20X coverage
Raw Error Rate

<5% (~0.5% for substitutions)

Consistent from 20-80% GC content of target DNA

Independent of Read Length and Template Size

Template Size 25 to 5,000 bases

1 >50 samples per run possible with molecular barcoding and sample multiplexing
2 The imaging system is designed for 1 Gigabase/hour throughput performance.
Actual sequencing performance is determined by chemistry efficiency and loading density
3 Routine usage specifications based on genomic DNA samples loaded at recommended concentrations and 30 Quad run (8 days)
4 Usable strands are defined at >25 base pairs in length and at the defined raw error rate

 

To view the complete specifications document click here (pdf).

 

 

 

Questions?
For information regarding the HeliScope™ Sequencer, or any of our products, call 877-2-HELICOS or send us a note.