What if you could obtain a read from each end of the same exact molecule of DNA — and do this on billions of strands in parallel? Taking advantage of the power of True Single Molecule Sequencing (tSMS)™, the HeliScope™ Single Molecule Sequencer can provide billions of paired-end reads from individual single molecules — without the tedious and costly molecular biology steps (cloning, circularization, digestion) required for traditional paired tag preparation. Using this powerful strategy, scientists can identify structural genomic variations such as inversions, translocations, amplifications and Alu repeats, and correlate them with genome regulation and disease.

Paired-end reads are easily obtained on the HeliScope Single Molecule Sequencer:

1. The genomic sample is digested/sheared.
2. Fragments within a desired size range are selected.
3. An adaptor sequence, containing a universal priming site, is ligated to the 5’ ends of the fragments.
4. Poly(A) tails are generated on the 3’ ends of the fragments.

The sample is now ready for paired-end sequencing.

		Paired-end Sequencing The modified templates are hybridized to the poly(T) surface in the HeliScope Flow Cell. pause sequence <<   |   1   |   2   |   3   |   4   |   5   |   6   |   7   |   >>

See some of the applications that can benefit from paired-end reads:

• Whole Genome Resequencing